abstract
antioxidant and increase lipid peroxidation can lead to oxidative damage to
cellular macro molecules leading to cancer. This study included 50 patients with
colon cancer were collected from (Al-Hussain Medical City/Kerbala, Digestive
and Liver Disease Education Hospital/ Medical City/ Baghdad and Oncology
Teaching Hospital / Medical City/ Baghdad) and 33 healthy people from ages
commensurate with patients aged.
The clinical characteristics of patients were documented, which included
age, gender, family history, stage of tumor, smoking, other disease, obesity and
drug. 32% of patients were younger and 68% were more than 41 years, 42% were
female and 58% were male, 64% had a family history of cancer, (6 %, 26 %, 60
% and 8 %) patients with stage of cancer from (Ⅰ to Ⅳ) respectively, 42% were
smokers, 50% patients with diabetes mellitus, 28% were obeses, 40% were
treated with chemotherapy and 82% were illiterate. The level of reduced
glutathione (GSH), catalase (CAT), ceruloplasmin oxidase (Cp oxidase),
malondialdehyde (MDA), zinc (Zn) and copper (Cu) were measured in sera of
patients with colon cancer and the control group.
The results showed a significant decrease (p<0.001) in the level of GSH
and Zn as well as a significant increase (p<0.001) in the level of MDA, Cu and
Cu/Zn in patients with colon cancer compared with the control group. Depending
on the Pearson’s correlation coefficients the results appeared a significant
positive correlation (r=0.5, p=0.02) between Cp oxidase and Cu. In addition,
there was a significant positive correlation between Cp oxidase (r=0.4, p=0.001)
and Cu/Zn ratio (r=0.23, p=0.04) in patients and the control group respectively.
Depending on the stage of colon cancer, GSH was a significant decrease
(p<0.001) in stage Ⅱ compared with stageⅠ, also there was a significant
decrease (p<0.05) in stage Ⅱ, Ⅲ and Ⅳ as compared with those of the control
group. In addition, catalase activity was lowered in stage Ⅰand Ⅳ as compared
II
with the control group, whereas MDA level was elevated in all stages of colon
cancer patients when compared with those of the control group, Cp oxidase was
significantly lowered (p<0.001) in stageⅠcomparing with the control group, but
the level of Cu and Cu/Zn was significantly increase (p<0.001) in all stages group
whereas there was a significant decrease (p<0.001) in the level of Zn as compared
with the control group.
The demographical study revealed a significant decrease (p<0.001) in the
activity of catalase and a significant increase (p<0.05) in the level of MDA in
male patients compared with the female. Smoker patients revealed a significant
decrease (p<0.001) in the levels of GSH and Zn and a significant increase (p<
0.001) in level of MDA and Cu/Zn ratio compared with the control group. Lipid
peroxidation was significant elevated (p<0.001) in patients who were treated
with chemotherapy drug. Also, this study showed a high proportion of diabetes
mellitus type 2 in patients with colon cancer compared with the other disease, in
addition the results were divided into three groups, group one represented
patients who had colon cancer only, group two patients had T2DM whereas
group three those represented patients who had other disease, T2DM and colon
cancer. The results were estimated by using ANOVA test, and the results
revealed a significant decrease (p<0.01) in the level of CAT among the three
groups. By using Pearson’s correlation coefficient, there was a significant
negative correlation (r= -0.3, p=0.02) between BMI and the activity of CAT, and
a significant positive correlation between BMI with MDA (r=0.3, p=0.04) and
with Cp oxidase (r=0.28, p=0.04) respectively.
These results indicate that free radicals and antioxidants play a n important
role in colon cancer patients and the chemotherapy drug is work to increase the
presence of free radicals in the body. Women has less colon cancer risk than m