Abstract

     60 males from white rat were used divided randomly for three main groups each one of them contained on 20 individuals of animals were treated by (2 , 4 & 6) doses from prostaglandin E₁(PGE₁).

     Each main group was divided randomly for 4 subgroups each one contained 5 animals. The subgroups treated by solution from 0.9% NaCl and (10µg/kg of body weight , 20 µg/kg of body weight and 30 µg/kg of body weight) from PGE₁.

   The weights before and after 24 hours from the last dose of treatment were measured, and also testes of animals were weighted after moved them and the histological sections were prepared for studying the effecting of the prostaglandin E₁on the mean spermatogenic cells and Sertoli cells and the percentage of damage and the changeables of mean diameters of seminiferous tubules were compared with control groups.

   The results were:-

   Significant increase (P<0.05) in the weight of animals that treated by 2 in all cocentrations and 4 doses from (10 , 30) µg/kg of body weight from PGE₁.

   The significant decrease had occurred in all mean weight of animals that treated by 6 doses The mean of testes weight was decreased significantly (P<0.05) in animals that treated by 6 doses from 10 µg/kg of body weight PGE₁.

   The mean of spermatogonia were decreased significantly in groups of animals that treated by 2 , 4 & 6 doses from 10 , (20 , 30) and (10 , 20 , 30) µg/kg of body weight PGE₁ in sequences .

   The significant decrease was noticed in mean spermatocytes of all animal’s groups that treated by PGE₁ except 2 groups that treated by 2 doses from (10 & 30) µg/kg of body weight PGE₁.

   All groups of animals that treated by PGE₁ suffered from significant decrease (P<0.05) in mean spermatids except the group that treated by 2 doses from 10 µg/kg of body weight PGE₁.

   Significant decrease (P<0.05) happened in mean Sertoli cells of animal’s groups that treated by 2 , 4 and 6 doses from 20 , 10 and (20 , 30) µg/kg of body weight PGE₁ in sequences.

   Some time significant increase and another time significant decrease had occurred in mean diameter of seminiferous tubules depend on doses and concentrations.

   The means percentage of seminiferous tubules that damaged increased significantly (P<0.05) in all animals that treated by PGE₁ depend on doses and concentrations .

     Different changeable of histopatholgical were found in testes of anomals depend on doses and concentrations of PGE₁.

   The aim of this study was to discover the effects and changes of testes tissue that caused by PGE₁ on spermatogenesis in adult white male rats.