Summary
The aim of this study was to investigate the effect of chronic exposed
of X-Ray on reproductive system in white female rats .
Twenty four adult of female rats were divided randomly into three
groups (8 animals / group), the first group considered as control group
(G1) , Second group (G2) were exposed to X-Ray (80kv), along one
meter/ daily for whole one month as well as the third group (G3) but for
two months, blood samples and tissue sections for ovary and uterus were
collected from the three treatments (groups) , after the determined period
was done and the next physiological parameters and The results of
current study present :-
Significant decrease ( P <0.05) in the number of (w.B.C , RBC,
PTL) and in level Hb concentration in the blood serum of exposed
animals of G2 and G3 as compared with G1. and Significant decrease
( P <0.05) in the number of (w.B.C , RBC, PTL) and in level Hb in G3
compared with G2.
significant increase (P <0.05) in the concentration of (TC, TG, LDL-C , VLDL-C) in the blood serum exposed animals G2 and G3 as
compared with G1. And significant increase (P <0.05) in the
concentration of (TC, TG, LDL, VLDL) in G3 compared with G2.
Significant decrease ( P <0.05) in the concentration of ( HDL-C, T–
protein) in the blood serum exposed animals G2 and G3 as compared
with G1. and Significant decrease ( P <0.05) in the concentration of
( HDL-C, T- protein) G3 compared with G2 .
significant decrease (P <0.05) in the concentration of (GSH) ) in the
blood serum exposed animals G2 and G3 as compared with G1. and
Significant decrease ( P <0.05) in the concentration of ( GSH) in G3
compared with G2 . also significant increase (P <0.05) in the
concentration of (MDA) in the blood serum of G2 and G3 as compared
with G1. And significant increase (P <0.05) in the concentration of
(MDA) in G3 compared with G2.
Significant decrease (P <0.05) in the concentration of Estrogen
hormone (E2), Progestrone hormone in the blood serum of exposed
animals of G2 and G3 as compared with G1. and no significant effect of
X-Ray in Follicle stimulating ( FSH ) and Luteinizing hormone ( LH) in
the blood serum of exposed animals of G2 and G3 as compared with G1.
The results of histological section of ovary exposed animals for one
month G2 ,to appear as blood congestion and inflammatory cells ,
comparing with control group G1 . while there was clear histological
changes in rats exposed for two month G3 include the occurrence of
destruction in Overian follicles, without oocytes , degeneration , fat
density and inflammatory cells in addition late effect of radiation injury
include nicrosis and fibrosis, comparing with control group G1.
The results of histological section of uterus in rat exposed to x-ray
for one month G1, thickness in the epithelial layer and hypertrophy in
some epithelial cells , and inflammatory cells comparing with control
group G1 , while the rat exposed for two month G3, the occurrence of
degeneration in the epithelial layer of uterus tissue , tearing or
laceration in uterian glands and nicrosis in stroma comparing with
control group G1
The results of Immunohistochemistrical expression proliferation
marker Ki67 ( tumer marker) in ovary tissue , was no significant increase
in gene expression of Ki67.
As conclude from the recent study that chronic exposed of X-Ray
radiation causes harmful effect on some blood and chemobiological
parameters and ovary , uterus tissue in female white rats.
