Summary

The aim of this study was to investigate the effect of chronic exposed

of X-Ray on reproductive system in white female rats .  

Twenty four   adult of female rats were divided randomly into three

groups (8 animals / group), the first group considered as control group

(G1) , Second group (G2) were exposed to X-Ray (80kv), along one

meter/ daily for whole one month as well as the third group (G3) but for

two months, blood samples and tissue sections for ovary and uterus were

collected from the three treatments (groups) , after the determined period

was done and the next physiological parameters and   The results of

current study present :-

Significant decrease ( P <0.05) in the number of (w.B.C , RBC,

PTL) and in level Hb concentration in the blood serum of   exposed

animals of   G2 and G3 as compared with G1. and Significant decrease              

( P <0.05) in the number of (w.B.C , RBC, PTL) and in level Hb in G3

compared with G2.

significant increase (P <0.05) in the concentration of (TC, TG, LDL-C , VLDL-C) in the blood serum exposed animals   G2 and G3 as

compared with G1. And significant increase (P <0.05) in the

concentration of (TC, TG, LDL, VLDL) in G3 compared with G2.

Significant decrease ( P <0.05) in the concentration of   ( HDL-C, T–

protein) in the blood serum exposed animals G2 and G3 as compared

with G1.   and Significant decrease ( P <0.05) in   the concentration of        

( HDL-C, T- protein) G3 compared with G2 .

significant decrease (P <0.05) in the concentration of (GSH) )   in the

blood serum exposed animals G2 and G3 as compared with G1. and

Significant decrease ( P <0.05) in   the concentration of ( GSH) in G3

compared with G2 . also significant increase (P <0.05) in the

concentration of (MDA) in the blood serum of G2 and G3 as compared

with G1. And significant increase (P <0.05) in the concentration of

(MDA) in G3 compared with G2.

Significant decrease (P <0.05) in the concentration of   Estrogen

hormone (E2), Progestrone hormone   in the blood serum of exposed

animals of G2 and G3 as compared with G1. and no significant effect of

X-Ray in Follicle stimulating ( FSH ) and Luteinizing hormone ( LH) in

the blood serum of exposed animals of G2 and G3 as compared with G1.

The results of histological section of ovary exposed animals for one

month G2 ,to appear as   blood congestion   and inflammatory cells ,

comparing with control group G1 . while there was clear histological

changes in rats exposed for two month G3 include the occurrence of

destruction in Overian follicles, without oocytes , degeneration , fat

density and inflammatory cells in addition late effect of radiation injury

include nicrosis and fibrosis, comparing with control group G1.

The results of histological section of uterus in rat exposed to x-ray

for one month G1, thickness in the epithelial layer and hypertrophy in

some epithelial cells , and inflammatory cells comparing with control

group G1 , while the rat exposed for two month G3, the occurrence of

degeneration in the epithelial layer of uterus tissue , tearing or

laceration   in uterian glands and nicrosis in stroma comparing with

control group G1

The results of   Immunohistochemistrical   expression proliferation

marker Ki67 ( tumer marker) in ovary tissue , was no significant increase

in gene expression of Ki67.  

As conclude from the recent study that chronic exposed of   X-Ray

radiation   causes harmful effect on some blood and chemobiological

parameters and ovary , uterus tissue in female white rats.