abstract

      This study involved isolation and identification bacterial causes of the urinary tract infection (UTI), as it was collected (65) urine sample from patients suffering from urinary tract, in the holy city of Karbala (Al-Hussein Hospital , children and women and obstetrics) for the period from October 2014 to March 2015 , these isolates  were cultured on selective and differential media and identified biochemicaly by using tapes Api 20 E , it was obtained bacteria Proteus mirabilis , E.coli Pseudomonas aeruginosa and Serratia marcescens (40, 20, 31.42,8.58) % of the total bacteria isolated ,respectiveily.

     Antibiotic susceptibility testing was done for the P.aeruginosa and P. mirabilis to determine the most effective against these isolates. Showed that the most effective   antibiotic is ciprofloxacin (CIP) and amikacin (AK) and imipenem (IPM) while most of the isolates were resistant to Ampicillin and Tetracycline then two most antibiotics , which resisted the two types of bacteria above.

     the minimum inhibitory concentration for three different antibiotics were study , and showed MIC values ​​of ciprofloxacin (0.25- 64) μg / ml.

While MIC values ​​ of amikacin (2 -128) μg / ml. While it was the imipenem ranging between (2-32) μg / ml.

     Some associated virulence factors of isolates were studied, it is found that the production of Hemolysin by Pseudomonas aeruginosa and proteus mirabilis was increased by (100)%, all isolates for two types of bacteria produced  for the enzyme  protease , also gave (100) %, a positive result for the production of biofilm. As the selected isolates show the ability to adhesion on epithelial cell surfaces at a rate between (17-51.66) cell / epithelial cell, as well as bacterial cell surface hydrophobicity test.

   As for the test swarming movement, the study demonstrated the ability of bacteria to swarm on enriched agar plate (1.5-2.0 ) % agar,as was noted that there is an inverse relationship between the amount of agar  and movement swarming fact agar solid hinder the movement of bacteria on the center of nutrient broth . Has also been an effective microbial antibiotics to three in the inhibition of growth and produce  virulence   factors in the two types of bacteria isolated . The results indicated that the antibiotics used in the study   its effectiveness against isolates studied and graduated effectiveness as follows:

Amikacin,Ciprofloxacin and Imipenem also showed antibiotic good effective in inhibiting virulence factors studied .